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Biochim Biophys Acta. 1995 Oct 25;1252(2):180-4.

Does the sulfhydryl or the adenine moiety of CoA enhance firefly luciferase activity?

Author information

1
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078-0454, USA.

Abstract

Light production by firefly luciferase is limited by product release resulting in flash kinetics. Several compounds (CoA, PPi, and nucleotides) transform the flash-form of light production into continuous light production. The sulfhydryl group of CoA is required; however, since nucleotides are also active, at least two mechanisms (sites) must exist.

PMID:
7578220
DOI:
10.1016/0167-4838(95)00150-s
[Indexed for MEDLINE]

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