Interleukin-1 and oncostatin M in combination promote the release of collagen fragments from bovine nasal cartilage in culture

Biochem Biophys Res Commun. 1995 Oct 4;215(1):377-85. doi: 10.1006/bbrc.1995.2476.

Abstract

Interleukin-1 (IL-1) and Oncostatin M (OM) induce a rapid and reproducible release of proteoglycan and collagen fragments from bovine nasal cartilage in culture. Over 90% of the total collagen was released by day 14 compared to a variable release with IL-1 alone. This release was accompanied by the appearance of collagenolytic activity in the medium that cleaved collagen specifically at the one quarter/three quarter position. Tissue inhibitor of metalloproteinases (TIMP-1) activity was low or absent in media from resorbing tissue. The breakdown of cartilage collagen could be prevented by the addition of BB94, a specific matrix metalloproteinase (MMP) inhibitor. These results suggest that T-cell/macrophage products within inflammed joints can interact with pro-inflammatory cytokines and lead to the rapid destruction of connective tissue collagen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cartilage / drug effects
  • Cartilage / metabolism*
  • Cattle
  • Collagen / metabolism*
  • Glycoproteins / metabolism
  • Glycosaminoglycans / metabolism
  • Interleukin-1 / administration & dosage
  • Interleukin-1 / pharmacology*
  • Kinetics
  • Metalloendopeptidases / antagonists & inhibitors
  • Nose
  • Oncostatin M
  • Peptides / administration & dosage
  • Peptides / pharmacology*
  • Phenylalanine / analogs & derivatives
  • Phenylalanine / pharmacology
  • Protease Inhibitors / pharmacology
  • Proteoglycans / metabolism
  • Thiophenes / pharmacology
  • Tissue Inhibitor of Metalloproteinases

Substances

  • Glycoproteins
  • Glycosaminoglycans
  • Interleukin-1
  • Peptides
  • Protease Inhibitors
  • Proteoglycans
  • Thiophenes
  • Tissue Inhibitor of Metalloproteinases
  • Oncostatin M
  • Phenylalanine
  • Collagen
  • batimastat
  • Metalloendopeptidases