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FEBS Lett. 1995 Sep 25;372(2-3):199-202.

Molecular cloning and functional expression in E. coli of a novel plant enzyme mediating zeta-carotene desaturation.

Author information

1
Biosynthesis Group, Fachbereich Biologie, J.W. Goethe Universit├Ąt, Frankfurt, Germany.

Abstract

We have cloned a cDNA from the plant Capsicum annuum which encodes a novel enzyme mediating the dehydrogenation of zeta-carotene and neurosporene to lycopene when expressed in E. coli cells accumulating zeta-carotene or neurosporene. This enzyme is unable to dehydrogenate either phytoene or lycopene. The deduced amino acid sequence suggests that this cDNA encodes a polypeptide whose mature size is ca. 59 kDa and which is synthesized as a precursor with a NH2-terminal extension resembling transit peptides for plastid targeting. Sequence comparison reveals 33-35% similarity with previously cloned plant or cyanobacterial phytoene desaturases. In contrast, only limited sequence similarity is found with a zeta-carotene desaturase from the cyanobacterium Anabaena.

PMID:
7556669
DOI:
10.1016/0014-5793(95)00978-i
[Indexed for MEDLINE]
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