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Biotechniques. 1995 Jul;19(1):122-4, 126-9.

Biotinylated hyaluronan as a probe for detection of binding proteins in cells and tissues.

Author information

1
Tufts University School of Medicine, Boston, MA, USA.

Abstract

A convenient and reliable method for preparing and using biotinylated hyaluronan for detection of hyaluronan-binding proteins is described. The biotinylated hyaluronan can be used to detect binding proteins in transblots after electrophoresis or as a histological probe for localization of binding proteins in cultured cells and tissue sections.

PMID:
7545407
[Indexed for MEDLINE]

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