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Biochem Mol Biol Int. 1995 May;36(1):195-202.

A new method of human cardiac troponin I and troponin T purification.

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Russian Center of Molecular Diagnostics and Medical Care, M. V. Lomonosov Moscow State University.


New rapid and effective procedure for simultaneous purification of human cardiac troponin I and troponin T has been developed. Affinity chromatography on immobilized monoclonal antitroponin I antibody C5 was used for purification of the whole troponin complex with a yield of 120 mg from 100 g of tissue. Isolated troponin I and troponin T (about 20 and 35 mg from 100 g tissue) were obtained by conventionally used ion-exchange chromatography. Antibody C5 recognizes conservative epitope of troponin I, therefore the method is applicable for purification of skeletal and cardiac troponin from a number of different animal species.

[Indexed for MEDLINE]

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