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Protein Expr Purif. 1995 Feb;6(1):25-32.

Nucleic acid affinity chromatography: preparation and characterization of double-stranded RNA agarose.

Author information

1
Department of Microbiology, Arizona State University, Tempe 85287-2701, USA.

Abstract

An improved method for the preparation of a double-stranded RNA-coupled resin is described. Periodate-oxidized double-stranded RNA, in the form of either purified reovirus double-stranded RNA or poly(rl). poly(rC), was covalently attached to agarose-adipic acid hydrazide. The resulting affinity resin had increased specificity for known double-stranded RNA binding proteins, as compared to a similar commercially available resin. The application of this resin in the purification and characterization of double-stranded RNA binding proteins is described.

PMID:
7538839
DOI:
10.1006/prep.1995.1004
[Indexed for MEDLINE]

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