Format

Send to

Choose Destination
Electrophoresis. 1994 Dec;15(12):1552-8.

Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension.

Author information

1
CEA-Laboratoire de Biologie, Mol├ęculaire du Cycle Cellulaire, INSERM U309, Grenoble.

Abstract

We describe a modification in the sample application mode for isoelectric focusing with immobilized pH gradients. Instead of being applied at the surface of the gel in a sample cup, the sample is introduced into the gel during the immobilized pH gradient strip rehydration step. This modification implies the use of low percentage gels (below 3.5% T) and specially designed, but simple, rehydration chambers. The main advantages are a uniform resolution without side effects and the possibility of handling large sample volumes (500 microL for a standard 3 x 160 x 0.5 mm strip), allowing micropreparative work (milligram samples) with a simple experimental design.

PMID:
7536671
DOI:
10.1002/elps.11501501223
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center