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J Med Virol. 1995 Jan;45(1):43-9.

Lack of augmenting effect of interferon-gamma on dengue virus multiplication in human peripheral blood monocytes.

Author information

1
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Thailand.

Abstract

The effect of interferon-gamma (IFN-gamma) on dengue virus multiplication in human peripheral blood monocytes was investigated. Enriched monocytes were treated with IFN-gamma and then infected with dengue virus type 2 either directly or in the presence of optimal infection-enhancing levels of antibodies. Pretreatment of monocytes from dengue-immune donors with 100 IU/ml of IFN-gamma caused 12- to 97-fold and 13- to 137-fold reduction of virus yields at 24 hr after infection in the absence and presence of an anti-flavivirus monoclonal antibody, respectively. IFN-gamma also diminished virus yields when infection of monocytes from a donor who lacked anti-dengue antibody was enhanced 40-fold. The percentage of infected monocytes in IFN-gamma-pretreated cultures was similarly reduced. Dominance of the antiviral effect of IFN-gamma in monocytes is in contrast to an augmenting effect previously observed in the promonocytic cell line U937.

PMID:
7536230
DOI:
10.1002/jmv.1890450109
[Indexed for MEDLINE]

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