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Neuron. 1995 Feb;14(2):211-5.

The jellyfish green fluorescent protein: a new tool for studying ion channel expression and function.

Author information

1
Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520.

Abstract

Two methods are described for using the jellyfish green fluorescent protein (GFP) as a reporter gene for ion channel expression. GFP fluorescence can be used to identify the transfected cells, and to estimate the relative levels of ion channel expression, in cotransfection experiments. A GFP-NMDAR1 chimera can be constructed that produces a functional, fluorescent receptor subunit. These methods should facilitate studies of ion channel expression, localization, and processing.

PMID:
7531985
DOI:
10.1016/0896-6273(95)90279-1
[Indexed for MEDLINE]
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