The E-selectin-ligand ESL-1 is a variant of a receptor for fibroblast growth factor

Nature. 1995 Feb 16;373(6515):615-20. doi: 10.1038/373615a0.

Abstract

E-SELECTIN is an inducible cell-adhesion molecule on endothelial cells, which mediates the binding of neutrophils and functions as a Ca(2+)-dependent lectin. We have recently identified a 150K glycoprotein as the major ligand for E-selectin on myeloid cells, using a recombinant antibody-like form of mouse E-selectin as an affinity probe. Here we report the isolation of a mouse complementary DNA for this E-selectin ligand (ESL-1). The predicted amino-acid sequence of ESL-1 is 94% identical (over 1,078 amino acids) to the recently identified chicken cysteine-rich fibroblast growth-factor receptor, except for a unique 70-amino-acid aminoterminal domain of mature ESL-1. Fucosylation of ESL-1 is imperative for affinity isolation with E-selectin-IgG. A fucosylated, recombinant antibody-like form of ESL-1, but not of L-selectin, supports adhesion of E-selectin-transfected Chinese hamster ovary cells. Antibodies against ESL-1 block the binding of mouse myeloid cells to E-selectin. ESL-1, with a structure essentially identical to that of a receptor, thus functions as a cell adhesion ligand of E-selectin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CHO Cells
  • Cell Adhesion Molecules / metabolism*
  • Chickens
  • Cloning, Molecular
  • Cricetinae
  • DNA, Complementary
  • E-Selectin
  • Fucose / metabolism
  • Glycoproteins / chemistry
  • Glycoproteins / genetics
  • Glycoproteins / isolation & purification*
  • Glycoproteins / metabolism
  • Humans
  • Immunoglobulin G / genetics
  • Immunoglobulin G / metabolism
  • Ligands
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / isolation & purification*
  • Membrane Glycoproteins / metabolism
  • Mice
  • Molecular Sequence Data
  • Neutrophils / metabolism
  • Precipitin Tests
  • Protein Binding
  • Receptors, Fibroblast Growth Factor / chemistry*
  • Receptors, Fibroblast Growth Factor / genetics
  • Receptors, Fibroblast Growth Factor / isolation & purification*
  • Receptors, Fibroblast Growth Factor / metabolism
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / isolation & purification
  • Receptors, Immunologic / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Sialoglycoproteins
  • Transfection

Substances

  • Cell Adhesion Molecules
  • DNA, Complementary
  • E-Selectin
  • Glycoproteins
  • Immunoglobulin G
  • Ligands
  • Membrane Glycoproteins
  • Receptors, Fibroblast Growth Factor
  • Receptors, Immunologic
  • Recombinant Fusion Proteins
  • Sialoglycoproteins
  • cysteine-rich fibroblast growth factor receptor
  • Fucose

Associated data

  • GENBANK/U28811
  • GENBANK/X84037