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Anal Biochem. 1994 Sep;221(2):303-5.

One-hour downward capillary blotting of RNA at neutral pH.

Author information

1
Division of Endocrinology, College of Medicine, University of Cincinnati, Ohio 45267-0547.

Abstract

This report describes a setup for the downward capillary blotting of RNA with the use of 10 x SSC as a transfer solution. The setup is composed of a stack of blotting papers, hybridization membrane, and agarose gel. Two layers of blotting paper connect the stack with two reservoirs containing transfer solution. Using this setup, blotting of RNA fragments (< 7.5 kb) can be completed in 1 h. If necessary, the blotting time can be expanded from 1 to 18 h without decrease in hybridization efficiency of RNA.

PMID:
7529006
DOI:
10.1006/abio.1994.1416
[Indexed for MEDLINE]

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