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Int J Biochem. 1994 Jun;26(6):813-23.

Oligoclonal beta-galactoside-binding immunoglobulins antigenically related to 14 kDa lectin in human serum and cerebrospinal fluid: purification and characterization.

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1
Laboratoire de Biochimie et Technologie des Protéines, Université Paris-Nord, Bobigny, France.

Abstract

1. An antiserum raised against a 14 kDa beta-galactoside specific lectin from human brain (HBL14) was used to probe blots from samples of serum and cerebrospinal fluid. The only HBL14-immunoreactive material detected was heavy and light chains of a beta-galactoside-binding IgG fraction (lectin-like IgG). 2. Lectin-like IgG, as well as IgG Fab fragments, compete with HBL14 for binding either to anti-HBL14 antibody or to a lactosyl polyacrylamide-based copolymer. 3. Purification of lectin-like IgG was obtained by affinity chromatography on immobilized rabbit anti-lectin immunoglobulins. The carbohydrate-binding specificity of the purified molecules was restricted to beta-Gal-containing structures and close to the HBL14 one.

PMID:
7520398
[Indexed for MEDLINE]
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