Format

Send to

Choose Destination
J Immunol Methods. 1994 Mar 29;170(1):15-25.

In vitro primary responses of human T cells to soluble protein antigens.

Author information

1
Department of Pathology and Microbiology, Medical School, University of Bristol, UK.

Abstract

The optimum culture conditions to support in vitro proliferative responses to conventional soluble protein antigens by human CD4+ T cells from healthy non-immunised donors have been determined. These responses were blocked by anti-HLA-DR monoclonal antibodies (mAbs) and were strictly dependent on the presence of antigen-presenting cells (APCs). Primary responses showed a reproducible pattern of proliferation kinetics which distinguished them from secondary in vitro T cells reactions. T cells specific for the sensitising antigen were recovered from primary cultures.

PMID:
7512606
DOI:
10.1016/0022-1759(94)90241-0
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center