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Anal Biochem. 1995 Sep 20;230(2):290-4.

Halophilic protein stabilization by the mild solubilizing agents nondetergent sulfobetaines.

Author information

1
Institut Laue-Langevin, Grenoble, France.

Abstract

In this work, experiments performed on pig heart and halophilic malate dehydrogenase as well as halophilic elongation factor Tu demonstrate a protein stabilization property from the recently described mild solubilizing agents nondetergent sulfobetaines. A practical application is given by the separation of halophilic bacteria elongation factor Tu and halophilic malate dehydrogenase by high-performance ion-exchange chromatography achieved at reduced salt levels without significant loss of activity.

PMID:
7503420
DOI:
10.1006/abio.1995.1476
[Indexed for MEDLINE]

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