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Electrophoresis. 1995 Jul;16(7):1087-9.

Sample preparation of human tumors prior to two-dimensional electrophoresis of proteins.

Author information

1
Department of Cell and Molecular Analysis, Karolinska Institute, Stockholm, Sweden.

Abstract

Sample preparation procedures are critical for obtaining high quality two-dimensional electrophoresis (2-DE) separations from clinical tumor materials. In this communication, we describe procedures which we have found satisfactory for preparing various tumor samples for 2-DE. By these procedures, tumor cells are enriched from serum proteins and contaminating stromal cells. Tumors can be prepared with good reproducibility to obtain high resolution using these techniques.

PMID:
7498151
DOI:
10.1002/elps.11501601184
[Indexed for MEDLINE]

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