Format

Send to

Choose Destination
Electrophoresis. 1995 Jun;16(6):948-51.

A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate-polyacrylamide gels.

Author information

1
Institute for Marine Biosciences, National Research Council of Canada, Halifax, N.S.

Abstract

A simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The method includes the incorporation of a water wash after a short fixation step in ethanol/ acetic acid and prior to glutaraldehyde cross-linking, which appears to be necessary for the high resolution staining of protein bands and the low background staining.

PMID:
7498141
DOI:
10.1002/elps.11501601159
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center