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Gene. 1995 Nov 7;165(1):143-4.

An IS903-based vector for transposon mutagenesis and the isolation of gene fusions.

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Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06510, USA.


A derivative of the IS903 transposon (Tn) is described that is capable of creating lacZ gene fusions upon transposition. It should find wide use as a tool for Tn mutagenesis in bacteria since it can be used both to generate mutants and to examine gene expression. The transposase-encoding gene (tnp) is located outside the Tn in the vector, thus Tn insertions into a genome are stably maintained in the absence of its cis-acting transposase (Tnp). The element carries a KmR gene allowing for the direct selection of transposition events in hosts that cannot support pBR322 plasmid replication and facilitating the subcloning of genes into which the Tn has inserted.

[Indexed for MEDLINE]

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