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Gene. 1995 Nov 7;165(1):141-2.

New plasmids carrying antibiotic-resistance cassettes.

Author information

1
Department of Biology, College of William and Mary, Williamsburg, VA 23188, USA.

Abstract

A series of new plasmid vectors is described that carry gene cassettes imparting resistance to the antibiotics chloramphenicol (CmR), kanamycin (KmR), tetracycline (TcR) and spectinomycin/streptomycin (Sp/SmR). The gene cassettes are symmetrically flanked by several restriction sites. In addition, several restriction sites that are normally found internal to the gene cassettes have been eliminated, thereby expanding the number of restriction enzymes available to excise an intact antibiotic-resistance gene. The gene cassettes are carried by high-copy-number plasmids that confer ampicillin resistance (ApR).

PMID:
7489905
DOI:
10.1016/0378-1119(95)00529-f
[Indexed for MEDLINE]

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