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Gene. 1995 Nov 7;165(1):127-30.

Green fluorescent protein production in the cellular slime molds Polysphondylium pallidum and Dictyostelium discoideum.

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Department of Molecular Biology, Princeton University, NJ 08544, USA.


The green fluorescent protein-encoding gene from Aequorea victoria has been cloned into several different transforming vectors and expressed in the cellular slime molds, Polysphondylium pallidum and Dictyostelium discoideum. We find that the protein is stable and non-toxic in both species, can be easily visualized in living and fixed specimens, and can be used to purify rare cells by fluorescence-activated cell sorting (FACS).

[Indexed for MEDLINE]

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