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Int J Obes Relat Metab Disord. 1995 Aug;19(8):544-9.

Reproducibility of 24-h energy expenditure, substrate utilization and spontaneous physical activity in obesity measured in a respiration chamber.

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Research Department of Human Nutrition, Royal Veterinary and Agricultural University, Copenhagen, Denmark.



Determination of within-subject day-to-day coefficients of variations (CV) of 24-h measurements of spontaneous physical activity (SPA), macronutrient oxidation, energy expenditure (EE) and heart rate (HR).


Two 24-h measurements in respiration chamber, separated by 14 days of strict weight stability.


22 healthy obese subjects, 15 females and seven males (age 27-64 y, BMI: 30-39 kg/m2).


SPA assessed by microwave radar, substrate oxidation and EE measured by indirect calorimetry and heart rate measured by telemetry.


For 24-h measurements CV was 0.8% for RQ, 6.7% for fat oxidation, 5.6% for carbohydrate oxidation, 14.5% for protein oxidation, and 4.3% for heart rate. SPA assessed by microwave radar was also reproducible (CV = 9.7%). Within-subject day-to-day coefficients of variations (CV) were 1.5 and 1.8% for 24-h EE and sleeping EE, and 4.1% for RMR. Both on day 1 and 14, 93.4% of the variation in 24-h EE could be explained by differences in fat-free mass (92.3 and 92.4%, respectively) and SPA (1.1 and 1.0%), respectively). Also the algorithm and correlation between 24-h RQ and 24-h energy balance were highly reproducible (r = 0.56, P < 0.01 and r = 0.48, P < 0.05).


Both macronutrient oxidation, SPA and EE in obese patients can be determined with high reproducibility in a respiration chamber.

[Indexed for MEDLINE]

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