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Biochem Biophys Res Commun. 1995 Nov 2;216(1):209-15.

DAN gene product has an affinity for Ni2+.

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1
Division of Biochemistry, Chiba Cancer Center Research Institute, Japan.

Abstract

Differential screening-selected gene aberrative in neuroblastoma (DAN) encodes a protein which possesses metal binding motifs. Glutathione S-transferase DAN fusion protein had an ability to bind to Ni(2+)-immobilized affinity resin. Truncation of the C-terminal region including a (HX)n repeat of DAN caused a loss of binding ability to the affinity resin, suggesting that this region is essential for Ni(2+)-binding. DAN produced in cultured rat cells also had an affinity for Ni2+. Cross-linking experiments demonstrated that the C-terminal region might function as a protein-protein interacting domain.

PMID:
7488091
DOI:
10.1006/bbrc.1995.2612
[Indexed for MEDLINE]
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