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Biochem Biophys Res Commun. 1995 Oct 13;215(2):682-90.

The carboxyl extensions of two rat ubiquitin fusion proteins are ribosomal proteins S27a and L40.

Author information

1
Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA.

Abstract

Two rat recombinant cDNAs were characterized; they encode fusion proteins that have at their NH2 terminus the conserved 76 amino acid ubiquitin and at their carboxyl terminus the extension ribosomal proteins S27a (80 amino acids and a molecular weight of 9,397) or L40 (52 amino acids and a molecular weight of 6,177). The fusion proteins are processed in a reticulocyte lysate to ubiquitin and either S27a or L40. Hybridization of cDNAs to digests of nuclear DNA suggests that there are 14 to 19 copies of the S27a, and 6 to 10 of the L40, genes. The mRNA for ubiquitin-S27a has about 700 nucleotides and ubiquitin-L40 about 650. Ribosomal proteins S27a and L40 contain zinc finger motifs of the C2-C2 variety. S27a and L40 are related to ribosomal proteins from other species.

PMID:
7488009
DOI:
10.1006/bbrc.1995.2518
[Indexed for MEDLINE]

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