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Mol Gen Genet. 1995 Oct 25;248(6):719-26.

Characterization of the let-653 gene in Caenorhabditis elegans.

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Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, B.C., Canada.


A mutation in the let-653 gene of Caenorhabditis elegans results in larval death. The lethal arrest is concurrent with the appearance of a vacuole anterior to the lower pharyngeal bulb. The position of the vacuole is consistent with a dysfunction of the secretory/excretory apparatus. Germline transformation rescue experiments were able to position the let-653 gene to two overlapping cosmid subclones. Sequence data generated from both cDNA and genomic DNA subclones indicated that let-653 encodes a mucin-like protein. Our characterization suggests that a mucin-like protein is essential for effective functioning of the secretory/excretory apparatus within C. elegans.

[Indexed for MEDLINE]

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