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Hum Genet. 1980;55(1):81-5.

Cytogenetic evidence for the absence of an inactivated X chromosome in a human female (XX) breast carcinoma cell line.

Abstract

Barr body staining procedures were applied directly to the chamber slide cultures of female amniotic cells. WI38 fibroblasts, normal female kidney cells, and a human breast carcinoma cell line, Elco. A high frequency of Barr bodies was found in all the normal female control cells; however, no Barr bodies were observed in the Elco cells. By trypsin G-banding analysis, two normal X chromosomes were identified in all Elco cells. The late DNA replication pattern of the cell line was then studied with the terminal BrdU pulse method. Both X chromosomes in the Elco cell line were found to be euchromatic with a characteristic R-banding pattern; no late-replicating X chromosome was observed. The absence of both a Barr body and a late-replicating heterocyclic X chromosome provides strong cytogenetic evidence that an inactivated X chromosome is absent in the human breast carcinoma cells bearing two X chromosomes.

PMID:
7450759
DOI:
10.1007/bf00329131
[Indexed for MEDLINE]

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