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Biochem J. 1978 Nov 15;176(2):365-70.

A rapid method for the preparation of relatively pure metabolically competent synaptosomes from rat brain.

Abstract

A rapid (less than 2h) method is described for the preparation of synaptosomes from rat brain by using a discontinuous Ficoll/sucrose gradient by a flotation technique. These synaptosomes are metabolically active and minimally (less than 5%) contaminated with 'free' mitochondria as judged by marker-enzyme assays and electron microscopy.

PMID:
743245
PMCID:
PMC1186243
DOI:
10.1042/bj1760365
[Indexed for MEDLINE]
Free PMC Article

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