The use of an animal model in the study of human diseases has proven a valuable tool for medical science. The main syndromes in human toxemia of pregnancy are hypertension, edema, and proteinuria. Because of their hypertension, pregnant spontaneously hypertensive rats (SHR) can be considered to represent a type of toxemia of pregnancy according to the classification of the Japan Society of Obstetrics and Gynecology. As no edema and only a small amount of proteinuria were observed in SHR, we induced massive proteinuria and edema by injecting pregnant SHR with puromycin. After this treatment, proteinuria of 100-300 mg/dl was continually observed throughout their life span, with pregnancy and delivery occurring 2-4 times. From our findings we have attempted to produce a model of human toxemia of pregnancy.