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Biophys J. 1981 Dec;36(3):575-88.

Analysis of data from the analytical ultracentrifuge by nonlinear least-squares techniques.

Abstract

Least-squares analysis of experimental data from the analytical ultracentrifuge is discussed in detail, with particular attention to the use of interference optics in studying nonideal self-associating macromolecular systems. Several samples are given that describe the application of the technique, the expected precision of the results, and some of its limitations. A FORTRAN IV computer program is available from the authors.

PMID:
7326325
PMCID:
PMC1327647
DOI:
10.1016/S0006-3495(81)84753-4
[Indexed for MEDLINE]
Free PMC Article

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