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Cell. 1978 Nov;15(3):935-43.

Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I.

Abstract

A simple and selective assay for monomeric and filamentous actin is presented, based on the inhibition of DNAase I by actin. In mixtures of monomeric and filamentous actin, only the monomeric form is measured as DNAase inhibitor. The total amount of actin in a sample can be determined after depolymerization of F actin with guanidine hydrochloride. The assay is rapid enough to detect changes in the polymerization state of actin in vitro over time intervals as short as 3 min. Data characterizing unpolymerized and filamentous actin pools in extracts of human platelets, lymphocytes and HeLa cells are presented.

PMID:
728995
DOI:
10.1016/0092-8674(78)90277-5
[Indexed for MEDLINE]

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