Some lysosomal enzymes (viz., acid DNase, acid RNase and beta-glucuronidase) were estimated in different parts of the rabbit Fallopian tube during different hours post coitum (p. c.). At estrus, alterations of acid RNase and beta-glucuronidase were observed in different anatomical segments of the Fallopian tube but acid DNase was undetectable. When these enzymes were compared at different hours p.c., it was noticed that when the ovum reaches ampullary (A), ampullary-isthmic junction (AIJ) and isthmic (I) segments of the Fallopian tube at the respective hours 14, 24 and 70, the acid DNase activity showed increased value in these parts when compared to their preceding groups. Acid RNase also showed similar type of pattern except that it was not altered at 14 hr p. c. At 144 hr p. c. both the enzymes had no significant alteration over 70 hr value, beta-glucuronidase, however, did not show this type of pattern in all the segments till 144 hr p. c. The increased activity of acid RNase and DNase in AIJ and I segments of the tube till 70 hr p. c. suggests the increased lysosomal activity in the tubal fluid produced by secretory cells. The possible involvement of these lysomal factors in the process of fertilization and preparation of ovum prior to implantation is suggested.