Format

Send to

Choose Destination
Biochemistry. 1981 Feb 17;20(4):833-40.

Phospholipid vesicle formation and transmembrane protein incorporation using octyl glucoside.

Abstract

Removal of detergent from mixed micelles of egg yolk phosphatidylcholine and octyl glucoside leads to formation of unilamellar phospholipid vesicles with a diameter of about 230 nm. The same procedure applied to mixed micelles containing the transmembrane protein glycophorin A, in addition to lipid and detergent, produces vesicles of the same size with glycophorin incorporated into the bilayer. The pure lipid vesicles are highly impermeable to both anions and cations, and incorporation of up to 220 molecules of glycophorin per vesicle has little effect on permeability.

PMID:
7213617
DOI:
10.1021/bi00507a028
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center