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Nucleic Acids Res. 1982 Apr 10;10(7):2439-51.

Induced hydrolytic activity of yeast phenylalanyl-tRNA synthetase by tRNAPhe-CC.

Abstract

"Induced hydrolysis" a new hydrolytic activity, was found by measuring AMP-production during aminoacylation of tRNAPhe-CCA by yeast phenylalanyl-tRNA synthetase in the presence of tRNAPhe-CC under conditions of low ionic strength at pH 8.5. Experiments using the elongation factor Tu . GTP provide evidence that transfer of phenylalanine to the tRNAPhe-CCA is followed by rapid hydrolysis in the presence of tRNAPhe-CC. A simple mechanism shows good agreement with the experimental data.

PMID:
7045811
PMCID:
PMC320621
[Indexed for MEDLINE]
Free PMC Article
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