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J Biol Chem. 1981 Aug 10;256(15):8092-101.

The amino acid sequence of elongation factor Tu of Escherichia coli. The large cyanogen bromide peptides.


The amino acid sequences of three large cyanogen bromide peptides, which comprise 289 of the 393 amino acids in Escherichia coli elongation factor Tu, have been determined. The peptides were digested with trypsin, Staphylococcus aureus protease, and chymotrypsin to give overlapping subfragments, which were separated by high performance liquid chromatography. Peptides were sequenced by solid-phase Edman degradation. Sequence analysis of these peptides completes the sequence of elongation factor Tu described in the companion article (Laursen, R. A., L'Italien, J. J., Nagarkatti, S. N., and Miller, D. L. (1981) J. Biol. Chem. 256, 8102-8109).

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