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Folia Microbiol (Praha). 1980;25(6):501-4.

Preparation of zymosan from yeast cell walls.

Abstract

Cell walls of the yeast Saccharomyces cerevisiae after disintegration and protoplasm removal by centrifugation and repeated washing were suspended in 0.5 M Na2HPO4, pH 7.8-8.0, as a 5% or 10% suspension, depending on the mode of heating. The suspension was boiled for 3 h, purified by repeated washing with water and ethanol and dried. The yield was approximately 1.8% of the starting amount of pressed commercial baker's yeast.

PMID:
7002749
DOI:
10.1007/bf02897218
[Indexed for MEDLINE]

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