Direct solid-phase 125I radioimmunoassay of serum cortisol

Clin Chem. 1978 Sep;24(9):1595-8.

Abstract

We report a solid-phase iodine- 125 radioimmunoassay for serum cortisol in which the interfering binding proteins are inactivated by a combination of reaction pH and 8-anilino-1-naphthalenesulfonic acid. The procedure is easy to perform and gives accurate and reliable results. The shift in pH for optimum binding of an antibody resulting from immobilization on a solid support, used to decrease cortisol binding competition in the present test, is potentially exploitable in other antibody and enzyme systems.

Publication types

  • Comparative Study

MeSH terms

  • Anilino Naphthalenesulfonates
  • Cross Reactions
  • Humans
  • Hydrocortisone / blood*
  • Iodine Radioisotopes
  • Radioimmunoassay / methods

Substances

  • Anilino Naphthalenesulfonates
  • Iodine Radioisotopes
  • Hydrocortisone