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J Immunol Methods. 1983 Aug 12;62(1):31-7.

Use of gelatin/plasma coated flasks for isolating human peripheral blood monocytes.

Abstract

A simple and efficient technique to purify human peripheral blood monocytes is described. This technique is based on the fact that monocytes have high affinity for fibronectin immobilized on a gelatin coated surface. Cell preparations obtained by the method described are more than 90% monocytes. The phenotype of these adherent cells was characterized with monoclonal antibodies.

PMID:
6875265
DOI:
10.1016/0022-1759(83)90107-2
[Indexed for MEDLINE]

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