Abstract
A simple and rapid technique is described for the removal of Triton X-100, deoxycholate, and cholate from protein solutions. The method involves a 2-min centrifugation of the sample on a Bio-Beads SM-2 bed prepared in a microcentrifuge tube and is suitable for multiple assays of 0.05- to 0.45-ml samples. Another advantage of this method is the high recovery of proteins without dilution of the sample.
MeSH terms
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Cholic Acid
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Cholic Acids / isolation & purification
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Deoxycholic Acid / isolation & purification
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Detergents / isolation & purification*
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Octoxynol
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Polyethylene Glycols / isolation & purification
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Proteins*
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Solutions
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Surface-Active Agents / isolation & purification*
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Ultracentrifugation
Substances
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Cholic Acids
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Detergents
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Proteins
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Solutions
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Surface-Active Agents
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Deoxycholic Acid
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Polyethylene Glycols
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Octoxynol
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Cholic Acid