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Histochemistry. 1981;71(1):1-16.

Histochemical demonstration of heavy metals. A revised version of the sulphide silver method suitable for both light and electronmicroscopy.

Abstract

The three steps of the sulphide silver method have been examined: 1) Transformation of metals to metal sulphides; 2) Fixation and embedding or freezing of the tissue for sectioning; and 3) Deposition of metallic silver on the metal sulphides in a physical developer. Based on the results, a revised method is described and discussed. It is particularly important 1) To maintain a sufficient but low concentration of sulphide ions during the perfusion; 2) To avoid using oxidating or acid fixatives; 3) To ensure low temperatures while embedding in paraffin or during polymerization of Epon; and 4) to use a slow-acting physical developer. Examples of the metal sulphide pattern from various tissues are presented.

PMID:
6785259
DOI:
10.1007/bf00592566
[Indexed for MEDLINE]

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