Location and release of TRH and 5-HT from amphibian skin

Cell Biol Int Rep. 1981 Feb;5(2):151-8. doi: 10.1016/0309-1651(81)90023-0.

Abstract

The occurrence and release of thyrotrophin-releasing hormone (TRH) and 5-hydroxytryptamine (5-HT) from amphibian skin have been described by previous investigators. In the present study, the precise location and site of release of TRH and 5-HT from the skin of Rana pipiens and Xenopus laevis have been examined using a combination of procedures including immunohistochemistry, HPLC, and radioimmunoassay. The results indicate that TRH is located specifically within the dermal glands of these species, and that both TRH and 5-HT are discharged from these glands following adrenergic stimulation. The origin and functional significance of these substances in amphibian skin granular glands are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Exocrine Glands / metabolism
  • Microscopy, Electron
  • Organ Culture Techniques
  • Rana pipiens / metabolism*
  • Serotonin / metabolism*
  • Skin / cytology
  • Skin / metabolism
  • Thyrotropin-Releasing Hormone / metabolism*
  • Xenopus laevis / metabolism*

Substances

  • Serotonin
  • Thyrotropin-Releasing Hormone