Send to

Choose Destination
See comment in PubMed Commons below
J Anat. 1980 May;130(Pt 3):603-15.

Changes in proliferation and surface morphology in the rat ileum in response to total parenteral nutrition.


To find out how the ileum adapts to total parenteral feeding, two experiments were performed. In the first experiment rats were given total intravenous feeding for 10 days. The animals were injected with tritiated thymidine (1 muCi/g body weight) 1 hour before being killed. Portions of the ileum were used for (1) radioautography, (2) analysis of the tissue DNA content, (3) specific activity of the DNA, and (4) scanning electron microscopy. The DNA content of ileum was decreased 72% while the specific activity of DNA was increased 289% in the i.v. fed rats. In the second experiment rats were given total intravenous feeding for 10 days. The animal were injected with colchicine (1 mg/kg body weight) 3 hours before being killed. The number of labelled cell nuclei per ileal crypt section was significantly decreased by parenteral feeding as was the number of colchicine collected metaphase figures. Light microscopy revealed the crypt and the villus height to be shorter and the number of goblet cells per unit surface area to be increased in parenterally fed rats as compared to those fed solid food orally. Enterocytes of the exfoliative zone from the ileal villi of rats fed solid food showed three distinct types of surface architecture whereas those from i.v. fed rats all possessed abundant microvilli. No bacteria were seen in ilea of i.v. fed animals but many were seen embedded in enterocytes from orally fed rats. Because the amount of DNA per cell is known to be constant, we concluded that the overall number of cells in the lieum decreased about 72% in the i.v. fed rats and that cell proliferation in the crypts, although significantly deceased, was still supporting an epithelial renewal process.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Support Center