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Genetics. 1982 Apr;100(4):565-77.

Determination of the order of gene function in the yeast nuclear division pathway using cs and ts mutants.

Abstract

Cold-sensitive (cs) and heat-sensitive (ts) conditional-lethal mutations that affect specifically the cell division cycle of budding yeast (Saccharomyces cerevisiae) were used to determine the order of gene function. Reciprocal temperature-shift experiments using cs-ts double mutants revealed a detailed order of function among genes whose execution points and mutant phenotypes are very similar. The data suggest that the nuclear branch of the overall cell-cycle pathway itself contains at least one branch.

PMID:
6749599
PMCID:
PMC1201832
[PubMed - indexed for MEDLINE]
Free PMC Article
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