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Am J Ophthalmol. 1984 Apr;97(4):488-95.

Quantitative analysis of wide-field endothelial specular photomicrographs.

Abstract

Samples from the center of a wide-field specular micrograph adequately assessed cell density and frequency distribution of cell areas. A preliminary analysis of the spread of values indicated whether an increased sample size was required. An algorithm determined how large a sample taken from the central photograph (three, five, or nine rectangles) was necessary to permit detection of a 10% change in cell area. This analysis of sample size also indicated that sampling by small-field specular microscopy was not representative of the surrounding square millimeter of corneal endothelium.

PMID:
6720820
DOI:
10.1016/s0002-9394(14)76133-1
[Indexed for MEDLINE]

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