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Biochim Biophys Acta. 1983 Oct 4;760(1):185-7.

A simple procedure for the synthesis of [32P]phosphoenolpyruvate via the pyruvate kinase exchange reaction at equilibrium.

Abstract

A one step procedure is presented for the preparation of [32P]phosphoenolpyruvate from [gamma-32P]ATP using pyruvate kinase. The reaction is carried out at chemical equilibrium and involves only an exchange of isotope between ATP and phosphoenolpyruvate. The initial phosphoenolpyruvate/ATP ratio in the reaction mixture determines the degree of 32P incorporation into phosphoenolpyruvate when isotopic equilibrium is achieved.

PMID:
6615882
DOI:
10.1016/0304-4165(83)90141-1
[Indexed for MEDLINE]

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