Isolation and purification of plasmids from Bacteroides fragilis using rubidium trichloroacetate density gradient centrifugation

E J Zöllner; C von Eichel-Streiber; F P Schwindling

doi:10.1007/BF00326072

Isolation and purification of plasmids from Bacteroides fragilis using rubidium trichloroacetate density gradient centrifugation

Mol Gen Genet. 1983;189(1):169-71. doi: 10.1007/BF00326072.

Authors

E J Zöllner, C von Eichel-Streiber, F P Schwindling

PMID: 6574305
DOI: 10.1007/BF00326072

Abstract

A rapid and easy final purification method is described for the isolation of plasmids from B. fragilis. Using RbTCA density gradient centrifugation in an airfuge ultracentrifuge ccc plasmid DNA can be separated from RNA, residual chromosomal DNA, linear and oc plasmid DNA. Pure ccc plasmid DNA is obtained from cultures of between 1 ml and 2 l in less than one day.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteroides fragilis / genetics*
Centrifugation, Density Gradient
DNA, Bacterial / isolation & purification*
Plasmids*
Rubidium
Trichloroacetic Acid

Substances

DNA, Bacterial
Trichloroacetic Acid
Rubidium