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FEBS Lett. 1984 Apr 9;169(1):35-9.

Localization of the initial steps in alkoxyphospholipid biosynthesis in glycosomes (microbodies) of Trypanosoma brucei.


Cell fractionation of Trypanosoma brucei cultured procyclic stages showed that the key enzyme of glycerol-ether lipid synthesis, dihydroxyacetone-phosphate acyltransferase (EC was exclusively associated with the microbody fraction. These organelles contained in addition 1-acyl glycerol-3-phosphate: NADP+ oxidoreductase (EC and acyl-CoA reductase and were capable of utilizing DHAP, but not G-3-P, as substrate for lysophosphatidic acid formation. It is concluded that in T. brucei the glycosomes are the exclusive site of the synthesis of precursors for glycerol-ether lipid synthesis and that they contain the entire pathway to form alkoxylipids from glycerol and acyl-CoA.

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