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Biochimie. 1984 Jul-Aug;66(7-8):531-7.

Actin purification from a gel of rat brain extracts.

Abstract

Actin, 99% pure, has been recovered from rat brain with a high yield (greater than 15 mg/100 g brain). We have shown that: 1. a low ionic strength extract from rat brain tissue is capable of giving rise to a gel; 2. actin is the main gel component and its proportion is one order of magnitude higher than in the original extract; 3. actin can be isolated from this extract by a three-step procedure involving gelation, dissociation of the gel in 0.6 M KCl, followed by one or two depolymerization-polymerization cycles.

PMID:
6529588
[Indexed for MEDLINE]
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