The importance of the corpus callosum for binocular interaction in areas 17 and 18 of the adult cat is still a matter of controversy, since its specific role in integrating information from the two eyes has been suggested by some and questioned by others. We have reanalyzed the problem by assessing binocular interaction for single neurons in areas 17 and 18 of adult cats submitted to section of the posterior two-thirds of the corpus callosum. In 5 cats this interhemispheric disconnection was performed from 10 days to 7 weeks before the electrophysiological recordings; in another cat callosal afferents to the recording sites were at first partially eliminated by an acute lesion of corresponding cortical zones in the other hemisphere, and thereafter completely interrupted by a posterior callosal section performed in the same recording session. Recordings were mainly aimed at the callosal zone of areas 17 and 18, which coincides with the border between these two areas and corresponds to visual field regions bordering the vertical meridian. Electrophysiological recordings were carried out in awake, unanesthetized animals in which all nociceptive pathways were previously interrupted by a midpontine pretrigeminal transection. The results indicate that the interhemispheric disconnection, whether acute or chronic, does not disrupt binocularity in areas 17 and 18; moreover, the analysis of the ocular dominance for binocular neurons did not reveal any imbalance between the inputs from the two eyes, since at all levels of eccentricity the majority of binocular neurons was equally activated by both eyes. Since in previous experiments on anesthetized cats, section of the corpus callosum apparently reduced binocular interaction in areas 17 and 18, we suggest that such an effect, which was lacking in our unanesthetized cats, was probably due to an interaction or summation between callosotomy and anesthesia.