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Biophys J. 1984 Aug;46(2):195-203.

Location of an extrinsic label in the primary and tertiary structure of bacteriorhodopsin.

Abstract

We located a heavy metal label, mercurilated phenylglyoxal, in both the primary sequence and in the tertiary structure of bacteriorhodopsin. This label modified arginines 225 and 227, which are on the COOH-terminal helix (G). In the projected electron potential difference map, the major site is close to the central inner helix. From this result we conclude that helix 1 could not be the COOH-terminal helix G. We tested the multiple isomorphous replacement method for obtaining phases for purple membrane by electron diffraction.

PMID:
6478034
PMCID:
PMC1435042
DOI:
10.1016/S0006-3495(84)84013-8
[Indexed for MEDLINE]
Free PMC Article

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