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J Biol Chem. 1980 Sep 25;255(18):8629-32.

The stereochemical course of phosphoric residue transfer during the myosin ATPase reaction.

Abstract

When adenosine 5'-(3-thiotriphosphate), stereospecifically labeled in the gamma position with 18O, was hydrolyzed in the presence of myosin subfragment 1 in 17O-enriched water, the product inorganic [16O,17O,18O]thiophosphate was chiral. The configuration of this product showed that the hydrolysis proceeds with inversion at the transferred phosphoric residue. This result suggests a direct, in-line hydrolysis mechanism for the ATPase.

PMID:
6447698
[Indexed for MEDLINE]
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