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Biophys J. 1983 Sep;43(3):383-6.

Fluorescent tandem phycobiliprotein conjugates. Emission wavelength shifting by energy transfer.

Abstract

A fluorescent tandem phycobiliprotein conjugate with a large Stokes shift was prepared by the covalent attachment of phycoerythrin to allophycocyanin. The efficiency of energy transfer from phycoerythrin to allophycocyanin in this disulfide-linked conjugate was 90%. A distinctive feature of this phycocyanin conjugate is the wide separation between the intense absorption maximum of phycoerythrin (epsilon = 2.4 x 10(6) cm-1 M-1 at 545 nm) and the fluorescence emission maximum of allophycocyanin (660 nm). Energy transfer from a donor to a covalently attached acceptor can be used to adjust the magnitude of the Stokes shift. Tandem phycobiliprotein conjugates can be used to advantage in fluorescence-activated cell sorting, fluorescence microscopy, and fluorescence immunoassay analyses.

PMID:
6414547
PMCID:
PMC1329306
DOI:
10.1016/S0006-3495(83)84361-6
[Indexed for MEDLINE]
Free PMC Article
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