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J Gen Microbiol. 1983 Apr;129(4):1239-42.

Penetration of oxygen into bacterial colonies.

Abstract

Previous estimates of the depth of oxygen penetration into bacterial colonies were made after measuring actual and potential respiration rates of whole colonies, or by calculation from kinetic values determined from the growth of bacteria in liquid culture. This paper reports the use of microelectrodes to measure oxygen penetration directly. Oxygen became undetectable 25-30 microns below the surface of a 120 microns deep, 18 h colony of Bacillus cereus. The colony was grown on a nutrient-rich agar medium incubated at 30 degrees C in a water-saturated atmosphere.

PMID:
6411858
DOI:
10.1099/00221287-129-4-1239
[Indexed for MEDLINE]

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